When using polarized light for example, a dark region may represent a free-of-stress and free-of-crystals material, or fully crystallized material, which may potentially experience mechanical stress switching to a non-polarized light would help to distinguish between the different cases. Furthermore, this study suggests that the ability to periodically switch between non-polarized light and polarized light is an essential feature of investigation. In addition, photoelasticity effects are used to demonstrate the development of residual stresses and the potential for stress relaxation above the glass transition temperature. Results of this study demonstrate polarized light as a visualization enhancement means, including the following effects: contaminants in the CPA solution, crystallization, fracture formation, thermal contraction, and solute precipitation. The current study aims at integrating polarized-light in the study of large-size cryopreservation, using the scanning cryomacroscope as a development platform. ![]() ![]() Cryomacroscopy is an effective means to observe physical events affecting cryopreservation success in large-size specimens.
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